Human Collagen Type II Alpha 1 ELISA Kit
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Name: Human Collagen Type II Alpha 1 ELISA Kit
Test type: Sandwich (quantitative)
Test principle
The microtiter plate provided in this kit has been pre-coated with an index-specific antibody. Standards or samples are then added to the appropriate microtiter plate wells with an index-specific biotin-conjugated antibody preparation. Next, horseradish peroxidase (HRP)-conjugated avidin is added to each well of the microplate and incubated. After adding the TMB Substrate Solution, only the wells containing the index, biotin-conjugated antibody, and enzyme-conjugated avidin will show a colour change. The enzyme-substrate reaction is terminated by adding sulfuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The index concentration in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Platform: microplate
Detection type: colorimetric
Example types: Serum, plasma and other biological fluids.
Sensitivity: 0.227ng/ml
Product range: 0.625 – 40ng/mL
Test time: 1 – 4.5 hours
Reactivity: Human
Synonyms
Collagen alpha-1 type I, CO1A1_HUMAN, COL1A1, COL2A1, COL3A1, COL4A1, COL5A1, alpha 1 collagen chain, alpha 1(I) collagen chain, alpha 1(II) collagen chain, alpha 1(III) collagen chain ), Collagen alpha 1(IV) chain, Collagen alpha 1(V) chain, Collagen alpha-1(I) chain, Collagen I + II + III + IV + V, Collagen I alpha 1 polypeptide, Collagen II alpha 1 polypeptide, Collagen IV alpha 1 polypeptide, Collagen type I alpha 1, Collagen type III alpha 1, Collagen type IV alpha 1, Collagen type V alpha 1, EDS4A
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.