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Topological tuning of DNA mobility in entangled solutions

Topological tuning of DNA mobility in entangled options of supercoiled plasmids Ring polymers in dense options are among the many most intriguing issues in polymer physics. Due to its pure incidence in round type, DNA has been extensively used as a proxy to check the elemental physics of ring polymers in several topological states. But, torsionally…

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Plasmid-mediated quinolone resistance genes

Excessive fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes amongst wholesome Korean folks in a metagenomic evaluation To characterize the carriage of antibiotic resistance genes (ARGs) within the intestine microbiome of wholesome people. Fecal carriage of ARGs was investigated in 61 wholesome people aged 30 to 59 years by means of entire metagenome sequencing…

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Biodistribution and safety studies of a bicistronic plasmid 

Biodistribution and security research of a bicistronic plasmid for nerve restore Background: Gene remedy is one of many promising approaches for regenerative drugs. Native and long-term expression of important progress elements permits to realize the specified therapeutic impact. Nonetheless, some features of extended utilization of genetic constructs encoding progress elements, resembling toxicity, mutagenicity, genotoxicity and talent to disseminate…

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Emergence and Expansion of a Carbapenem-Resistant

Emergence and Enlargement of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Related to Plasmid-Borne bla KPC-2 and Virulence-Associated Genes Pseudomonas aeruginosa is a significant opportunistic pathogen and one of many main bacterial species inflicting well being care-associated infections. Carbapenems are the best antimicrobial brokers for the therapy of extreme infections attributable to P. aeruginosa Nonetheless, our latest surveillance demonstrated that the prevalence of…

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Native conjugative plasmid confers potential selective

A local conjugative plasmid confers potential selective benefits to plant growth-promoting Bacillus velezensis pressure GH1-13 The conjugative plasmid (pBV71) presumably confers a selective benefit to Bacillus velezensis pressure GH1-13, though a selective marker gene is but to be recognized. Right here we present that few non-mucoid wild-type GH1-13 cells are spontaneously transformed to mucoid variants with or…

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Determination of incompatibility group plasmids

Determination of incompatibility group plasmids and copy number of the bla NDM-1 gene in carbapenem-resistant Klebsiella pneumoniae strains recovered from different hospitals in Kerman, Iran Introduction. New Delhi metallo-β-lactamase (NDM)-producing Klebsiella pneumoniae has become a serious global health concern. Hypothesis/Gap Statement. Due to the high genetic diversity among NDM-positive K. pneumoniae, we need further surveillance and studies to better understand the relationships between them. In addition, the coexistence…

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Cloning Vectors

Cloning vectors are plasmids (extrachromosomal circular DNA molecules that replicate independently of nuclear DNA) that carry inserted DNA fragments, typically a gene, that we want to introduce or express in the host. In order to be inserted, it is necessary to cut the vector with restriction enzymes and once it is introduced it is renamed: recombinant vector.…

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Community structure of diatoms present in the surface sediments of eight Andean lakes of central Chile

In this work, the community structure of diatoms deposited in the surface sediments of eight Chilean Andean lakes located between 32 ° 49 ‘and 38 ° 48’ S was analyzed. A total of 99 diatom taxa were found, distributed in 48 genera. Of the total taxa, 74 were identified at the species level, all of them…

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DIATOMACEOUS EARTH

Tierra de Diatomeas is a project by Susana, Inés Cámara Leret and María Boto Ordóñez whose objective is to investigate the relationship between the human being and the Castilian-Leonese geographic parcels. This research is part of a collaboration with the Diatomology Laboratory of the University of León, using diatoms as a metaphor to create a…

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Diatoms: the microscopic algae and “lungs” of the Earth that you should know

For many, it may come as a surprise, but the truth is that most of the annual oxygen production on our planet – between 50% and 85% – does not come from terrestrial forests, but from the ocean. Phytoplankton, a set of millions of microorganisms that are present in the waters that surround the five continents,…

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