Emergence and Expansion of a Carbapenem-Resistant
Emergence and Enlargement of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Related to Plasmid-Borne bla KPC-2 and Virulence-Associated Genes Pseudomonas aeruginosa is a significant opportunistic pathogen and one of many main bacterial species inflicting well being care-associated infections. Carbapenems are the best antimicrobial brokers for the therapy of extreme infections attributable to P. aeruginosa Nonetheless, our latest surveillance demonstrated that the prevalence of…
Read More
Native conjugative plasmid confers potential selective
A local conjugative plasmid confers potential selective benefits to plant growth-promoting Bacillus velezensis pressure GH1-13 The conjugative plasmid (pBV71) presumably confers a selective benefit to Bacillus velezensis pressure GH1-13, though a selective marker gene is but to be recognized. Right here we present that few non-mucoid wild-type GH1-13 cells are spontaneously transformed to mucoid variants with or…
Read More
Determination of incompatibility group plasmids
Determination of incompatibility group plasmids and copy number of the bla NDM-1 gene in carbapenem-resistant Klebsiella pneumoniae strains recovered from different hospitals in Kerman, Iran Introduction. New Delhi metallo-β-lactamase (NDM)-producing Klebsiella pneumoniae has become a serious global health concern. Hypothesis/Gap Statement. Due to the high genetic diversity among NDM-positive K. pneumoniae, we need further surveillance and studies to better understand the relationships between them. In addition, the coexistence…
Read More
Cloning Vectors
Cloning vectors are plasmids (extrachromosomal circular DNA molecules that replicate independently of nuclear DNA) that carry inserted DNA fragments, typically a gene, that we want to introduce or express in the host. In order to be inserted, it is necessary to cut the vector with restriction enzymes and once it is introduced it is renamed: recombinant vector.…
Read More
Community structure of diatoms present in the surface sediments of eight Andean lakes of central Chile
In this work, the community structure of diatoms deposited in the surface sediments of eight Chilean Andean lakes located between 32 ° 49 ‘and 38 ° 48’ S was analyzed. A total of 99 diatom taxa were found, distributed in 48 genera. Of the total taxa, 74 were identified at the species level, all of them…
Read More
DIATOMACEOUS EARTH
Tierra de Diatomeas is a project by Susana, Inés Cámara Leret and María Boto Ordóñez whose objective is to investigate the relationship between the human being and the Castilian-Leonese geographic parcels. This research is part of a collaboration with the Diatomology Laboratory of the University of León, using diatoms as a metaphor to create a…
Read MoreDiatoms: the microscopic algae and “lungs” of the Earth that you should know
For many, it may come as a surprise, but the truth is that most of the annual oxygen production on our planet – between 50% and 85% – does not come from terrestrial forests, but from the ocean. Phytoplankton, a set of millions of microorganisms that are present in the waters that surround the five continents,…
Read MoreDiatomaceous earth is a versatile product and 100% natural, for animals and plants
The diatomaceous earth is a mineral extracted from the diatomite, which is a siliceous sedimentary rock formed by the accumulation of fossil diatoms in lakes and seas of the past. This sedimentary rock is easily extractable and sprayable and currently has a large number of uses both in industrial processes and in agriculture. The diatoms are unicellular algae microscopic belonging class of Bacilonophyta and constitute a…
Read MoreTransformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
A bacterial (neo) gene conferring resistance to neomycin-kanamycin antibiotics has been inserted into SV40 hybrid plasmid vectors and introduced into cultured mammalian cells by DNA transfusion. While normal cells die with the antibiotic G418, those that acquire and express neo continue to grow in the presence of G418. In the course of selection, neo DNA…
Read More
Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBAD Promoter
We have constructed a series of plasmid vectors (pBAD vectors) that contain the PBAD promoter of the Arab and operon (arabinose) and the gene that encodes the positive and negative regulator of this promoter, araC. Using the phone gene and phoA fusions to monitor expression in these vectors, we show that the induction/repression ratio can…
Read More