• May 7, 2021

Cloning Vectors

Cloning vectors are plasmids (extrachromosomal circular DNA molecules that replicate independently of nuclear DNA) that carry inserted DNA fragments, typically a gene, that we want to introduce or express in the host. In order to be inserted, it is necessary to cut the vector with restriction enzymes and once it is introduced it is renamed: recombinant vector.…

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Community structure of diatoms present in the surface sediments of eight Andean lakes of central Chile

In this work, the community structure of diatoms deposited in the surface sediments of eight Chilean Andean lakes located between 32 ° 49 ‘and 38 ° 48’ S was analyzed. A total of 99 diatom taxa were found, distributed in 48 genera. Of the total taxa, 74 were identified at the species level, all of them…

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DIATOMACEOUS EARTH

Tierra de Diatomeas is a project by Susana, Inés Cámara Leret and María Boto Ordóñez whose objective is to investigate the relationship between the human being and the Castilian-Leonese geographic parcels. This research is part of a collaboration with the Diatomology Laboratory of the University of León, using diatoms as a metaphor to create a…

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Diatoms: the microscopic algae and “lungs” of the Earth that you should know

For many, it may come as a surprise, but the truth is that most of the annual oxygen production on our planet – between 50% and 85% – does not come from terrestrial forests, but from the ocean. Phytoplankton, a set of millions of microorganisms that are present in the waters that surround the five continents,…

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Diatomaceous earth is a versatile product and 100% natural, for animals and plants

The diatomaceous earth is a mineral extracted from the diatomite, which is a siliceous sedimentary rock formed by the accumulation of fossil diatoms in lakes and seas of the past. This sedimentary rock is easily extractable and sprayable and currently has a large number of uses both in industrial processes and in agriculture. The diatoms are unicellular algae microscopic belonging class of Bacilonophyta and constitute a…

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Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

A bacterial (neo) gene conferring resistance to neomycin-kanamycin antibiotics has been inserted into SV40 hybrid plasmid vectors and introduced into cultured mammalian cells by DNA transfusion. While normal cells die with the antibiotic G418, those that acquire and express neo continue to grow in the presence of G418. In the course of selection, neo DNA…

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Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBAD Promoter

We have constructed a series of plasmid vectors (pBAD vectors) that contain the PBAD promoter of the Arab and operon (arabinose) and the gene that encodes the positive and negative regulator of this promoter, araC. Using the phone gene and phoA fusions to monitor expression in these vectors, we show that the induction/repression ratio can…

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