
Emergence and Expansion of a Carbapenem-Resistant
kathryn
- 0
Emergence and Enlargement of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Related to Plasmid-Borne bla KPC-2 and Virulence-Associated Genes
Pseudomonas aeruginosa is a significant opportunistic pathogen and one of many main bacterial species inflicting well being care-associated infections. Carbapenems are the best antimicrobial brokers for the therapy of extreme infections attributable to P. aeruginosa Nonetheless, our latest surveillance demonstrated that the prevalence of carbapenem-resistant P. aeruginosa (CRPA) reached 38.67% in Zhejiang, China. By analyzing CRPA isolates collected from sufferers from 2006 to 2018, we discovered that 33% of CRPA isolates carried the gene bla KPC-2, which conferred high-level resistance to carbapenems and different β-lactams. Specifically, a CRPA clone, ST463 (sequence kind 463), emerged and has grow to be the predominant CRPA clone among the many inhabitants.
Genome sequencing demonstrated that ST463 growth was related with plasmid-borne bla KPC-2 The cell aspect flanking bla KPC-2, the sort IV secretion system, and the profitable growth of clone ST463 may need additional favored bla KPC-2 unfold in P. aeruginosa Molecular clock evaluation dated the emergence of clone ST463 to round 2007. Genome-wide affiliation evaluation confirmed that 567 genes have been related to clone ST463, together with a number of identified virulence genes associated to the biosynthesis of lipooligosaccharide (LOS) O-antigens and exotoxin. These findings point out that ST463 is increasing with plasmid-borne bla KPC-2 and virulence-related genes in nosocomial infections, and shut surveillance must be undertaken sooner or later.
IMPORTANCE Well being care-associated infections, often known as nosocomial infections, are essentially the most frequent opposed occasions in well being care supply worldwide, inflicting excessive charges of morbidity and mortality and excessive well being care prices. Pseudomonas aeruginosa is among the main bacterial species inflicting well being care-associated infections. Carbapenems are the best antimicrobial brokers for the therapy of its extreme infections. Nonetheless, the prevalence of carbapenem-resistant P. aeruginosa (CRPA) has been rising quickly in recent times, and our surveillance demonstrated that the prevalence of CRPA reached 38.67% in Zhejiang, China. Genome sequencing of CRPA isolates over a decade confirmed {that a} CRPA clone (ST463) emerged not too long ago. The clone is extremely immune to β-lactams, together with carbapenems, and fluoroquinolones. Genome-wide affiliation evaluation confirmed that the clone expanded with virulence-related genes and the plasmid-borne carbapenem-resistant gene bla KPC-2 These findings are of serious public well being significance, as the knowledge will facilitate the management and minimization of CRPA nosocomial infections.

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Full genome sequence of marine Roseobacter lineage member Monaibacterium sp. ALG8 with six plasmids remoted from seawater round brown algae
Monaibacterium sp. ALG8 (=MCCC 1 Ok04733) was remoted from seawater round brown algae. The genome of Monaibacterium sp. ALG8 was sequenced, one round 3,036,380 bp chromosome and 6 round plasmids starting from 12,229 to 151,263 bp have been discovered after meeting. The outcomes of genomic annotation confirmed that Monaibacterium sp. ALG8 lacks the flexibility to degrade alginate, indicating its ecological position is probably not instantly associated to the degradation of brown algae. The comparability of genomic options within the plasmids confirmed that the majority of those plasmids, besides pALG4, have been horizontally recruited from donors, not ancestors. Primarily based on predicted features, the existence of plasmids might present pressure ALG8 with benefits together with nitrate discount, tolerance of osmotic stress by way of glycine betaine, resistance to heavy steel stress equivalent to mercury and cobalt, degradation of benzoate metabolites equivalent to p-cumate, transformation of the swim-or-stick way of life and enchancment of the immune system with two CRISPR-Cas methods.
This research offers proof for the carbon metabolic patterns of Monaibacterium sp. ALG8 and predicts the features and donors of six plasmids on this pressure, broadening our understanding of the ecological roles of micro organism within the surroundings round brown algae and the features and evolutionary patterns of plasmids in marine Roseobacter lineage members.
Genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and curing of the endogenous plasmid pBV01
Goals: To assemble a genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and determine the origin aspect in an endogenous plasmidpBV01 for curing pBV01 by plasmid incompatibility.
Outcomes: A plasmid pUBC01 was constructed, after which an electrotransformation system for B. velezensis NSZ-YBGJ001 was developed, which reached ~ 1000 transformants per microgram of pUBC01 DNA. Moreover, a 7276-bp round plasmid pBV01 with a G + C content material of 37.5% was remoted from B. velezensis NSZ-YBGJ001 and analyzed by way of sequence evaluation. To remedy pBV01, an incompatible plasmid pBV02 harboring the replication aspect of pBV01 was developed and functionally replicated in each Bacillus subtilis WB600 and B. velezensis NSZ-YBGJ001. pBV01 was cured by way of introduction of pBV02 into B. velezensis NSZ-YBGJ001 after serial subculturing for roughly 40 generations. Lastly, one other plasmid, pBV03, was constructed primarily based on pBV-ori, and exogenous genes in pBV03 could possibly be effectively expressed in B. subtilis.
Conclusions: The outcomes of this research, together with the genetic transformation system, plasmid-curing technique, and exogenous gene expression, will help genetic manipulation of B. velezensis to advertise its software in biocontrol and trade.
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We present that the SMLP technique has a higher benefit than the traditional strategies examined on this research, and this technique might be utilized to substitution, deletion, and insertion mutations for each giant and small plasmids in addition to the meeting of three fragments from PCR reactions. Altogether, the SMLP technique is easy, efficient, and helpful to the laboratories that require finishing the mutagenesis of enormous plasmids.
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Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit |
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Pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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Rat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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Rat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E02C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E01C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E01C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E01C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E09C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E09C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E09C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Phospholipid transfer Antibody (Biotin) |
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20-abx105774 | Abbexa |
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Phospholipid transfer Antibody (FITC) |
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20-abx107192 | Abbexa |
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Phospholipid transfer Antibody (HRP) |
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20-abx108612 | Abbexa |
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Phospholipid transfer Polyclonal Antibody |
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42297-100ul | SAB | 100ul | EUR 333 |
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E05C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E05C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E05C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit |
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GA-E1532HM-48T | GenAsia Biotech | 48T | EUR 289 |
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit |
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GA-E1532HM-96T | GenAsia Biotech | 96T | EUR 466 |
Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit |
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201-12-1516 | SunredBio | 96 tests | EUR 440 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit |
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QY-E03917 | Qayee Biotechnology | 96T | EUR 361 |
Phosphatidylcholine Transfer Protein (PCTP) Antibody |
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20-abx302561 | Abbexa |
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Glycolipid Transfer Protein (GLTP) Antibody |
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20-abx303992 | Abbexa |
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SuperBlot Western Transfer buffer 10X |
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DG-WEST | Bio Basic | 500ml | EUR 113.8 |
Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx131266 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx131267 | Abbexa |
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Glycolipid Transfer Protein (GLTP) Antibody |
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20-abx128414 | Abbexa |
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Glycolipid Transfer Protein (GLTP) Antibody |
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20-abx112809 | Abbexa |
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Phosphatidylcholine Transfer Protein (PCTP) Antibody |
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20-abx114433 | Abbexa |
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Phosphatidylinositol Transfer Protein, alpha Antibody |
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20-abx114447 | Abbexa |
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Phosphatidylinositol Transfer Protein, beta Antibody |
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20-abx114448 | Abbexa |
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Phosphatidylcholine Transfer Protein (PCTP) Antibody |
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20-abx003727 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx004303 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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abx033529-400ul | Abbexa | 400 ul | EUR 523 |
Phospholipid Transfer Protein (PLTP) Antibody |
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abx033529-80l | Abbexa | 80 µl | EUR 286 |
Phosphatidylcholine Transfer Protein (PCTP) Antibody |
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abx028941-400ul | Abbexa | 400 ul | EUR 523 |
Phosphatidylcholine Transfer Protein (PCTP) Antibody |
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abx028941-80l | Abbexa | 80 µl | EUR 286 |
Phosphatidylinositol Transfer Protein, beta Protein |
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20-abx260804 | Abbexa |
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Phosphatidylinositol Transfer Protein, alpha Protein |
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20-abx260827 | Abbexa |
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Glycolipid Transfer Protein (GLTP) Protein |
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20-abx262570 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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abx146426-100ug | Abbexa | 100 ug | EUR 391 |
Phosphatidylcholine Transfer Protein (PCTP) Antibody |
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abx236230-100ug | Abbexa | 100 ug | EUR 551 |
Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx212015 | Abbexa |
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Glycolipid Transfer Protein (GLTP) Antibody |
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abx233495-100ug | Abbexa | 100 ug | EUR 509 |
Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx142148 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx178016 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx178017 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx174059 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx174060 | Abbexa |
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Xpert Native Transfer Buffer(10X) |
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P9001-050 | GenDepot | 500ml | EUR 93 |
Xpert Native Transfer Buffer(10X) |
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P9001-100 | GenDepot | 1L | EUR 138 |
Bovine Glycolipid transfer protein (GLTP) |
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1-CSB-YP009539BO | Cusabio |
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Description: Recombinant Bovine Glycolipid transfer protein(GLTP) expressed in Yeast |
Human Phospholipid transfer protein (PLTP) |
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1-CSB-YP018212HU | Cusabio |
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Description: Recombinant Human Phospholipid transfer protein(PLTP) expressed in Yeast |
Bovine Glycolipid transfer protein (GLTP) |
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1-CSB-EP009539BO | Cusabio |
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Description: Recombinant Bovine Glycolipid transfer protein(GLTP) expressed in E.coli |
Human Phospholipid transfer protein (PLTP) |
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1-CSB-EP018212HU | Cusabio |
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Description: Recombinant Human Phospholipid transfer protein(PLTP) expressed in E.coli |
Recombinant Glycolipid Transfer Protein (GLTP) |
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4-RPC502Hu01 | Cloud-Clone |
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Description: Recombinant Human Glycolipid Transfer Protein expressed in: E.coli |
Recombinant Phospholipid Transfer Protein (PLTP) |
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4-RPC728Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Phospholipid Transfer Protein expressed in: E.coli |
Recombinant Phospholipid Transfer Protein (PLTP) |
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4-RPC728Ra01 | Cloud-Clone |
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Description: Recombinant Rat Phospholipid Transfer Protein expressed in: E.coli |
Phospholipid transfer Polyclonal Conjugated Antibody |
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C42297 | SAB | 100ul | EUR 397 |