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Emergence and Expansion of a Carbapenem-Resistant

Emergence and Enlargement of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Related to Plasmid-Borne bla KPC-2 and Virulence-Associated Genes

Pseudomonas aeruginosa is a significant opportunistic pathogen and one of many main bacterial species inflicting well being care-associated infections. Carbapenems are the best antimicrobial brokers for the therapy of extreme infections attributable to P. aeruginosa Nonetheless, our latest surveillance demonstrated that the prevalence of carbapenem-resistant P. aeruginosa (CRPA) reached 38.67% in Zhejiang, China. By analyzing CRPA isolates collected from sufferers from 2006 to 2018, we discovered that 33% of CRPA isolates carried the gene bla KPC-2, which conferred high-level resistance to carbapenems and different β-lactams. Specifically, a CRPA clone, ST463 (sequence kind 463), emerged and has grow to be the predominant CRPA clone among the many inhabitants.

Genome sequencing demonstrated that ST463 growth was related with plasmid-borne bla KPC-2 The cell aspect flanking bla KPC-2, the sort IV secretion system, and the profitable growth of clone ST463 may need additional favored bla KPC-2 unfold in P. aeruginosa Molecular clock evaluation dated the emergence of clone ST463 to round 2007. Genome-wide affiliation evaluation confirmed that 567 genes have been related to clone ST463, together with a number of identified virulence genes associated to the biosynthesis of lipooligosaccharide (LOS) O-antigens and exotoxin. These findings point out that ST463 is increasing with plasmid-borne bla KPC-2 and virulence-related genes in nosocomial infections, and shut surveillance must be undertaken sooner or later.

IMPORTANCE Well being care-associated infections, often known as nosocomial infections, are essentially the most frequent opposed occasions in well being care supply worldwide, inflicting excessive charges of morbidity and mortality and excessive well being care prices. Pseudomonas aeruginosa is among the main bacterial species inflicting well being care-associated infections. Carbapenems are the best antimicrobial brokers for the therapy of its extreme infections. Nonetheless, the prevalence of carbapenem-resistant P. aeruginosa (CRPA) has been rising quickly in recent times, and our surveillance demonstrated that the prevalence of CRPA reached 38.67% in Zhejiang, China. Genome sequencing of CRPA isolates over a decade confirmed {that a} CRPA clone (ST463) emerged not too long ago. The clone is extremely immune to β-lactams, together with carbapenems, and fluoroquinolones. Genome-wide affiliation evaluation confirmed that the clone expanded with virulence-related genes and the plasmid-borne carbapenem-resistant gene bla KPC-2 These findings are of serious public well being significance, as the knowledge will facilitate the management and minimization of CRPA nosocomial infections.

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SpinCol Plasmid Miniprep Kit From Bacterial cells (Teaching)

52166-1 50 expt. Kit
EUR 60.64
Description: Part E

Plasmid DNA Isolation Solution I

10760028-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution I

10760028-2 500 mL
EUR 45.3

Plasmid DNA Isolation Solution II

10760029-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution II

10760029-2 500 mL
EUR 37.89

Plasmid DNA Isolation Solution III

10760030-1 250 mL
EUR 28.07

Plasmid DNA Isolation Solution III

10760030-2 500 mL
EUR 43

QC Buffer for Plasmid DNA Isolation

10760036-1 250 mL
EUR 25.45

QC Buffer for Plasmid DNA Isolation

10760036-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation

10760037-1 250 mL
EUR 24.44

QF Buffer for Plasmid DNA Isolation

10760037-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation

10760037-3 1 L
EUR 76.87

QBT Buffer for Plasmid DNA Isolation

10760035-1 250 mL
EUR 34.22

QBT Buffer for Plasmid DNA Isolation

10760035-2 500 mL
EUR 49.13

QBT Buffer for Plasmid DNA Isolation

10760035-3 1 L
EUR 71.93

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412018-10Columns 10Columns
EUR 160

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412018-250Columns 250Columns
EUR 835

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412018-50Columns 50Columns
EUR 295

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412018-5x250Columns 5x250Columns
EUR 3860

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412019-1x96DeepWellPlate 1x96DeepWellPlate
EUR 390

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412019-24x96DeepWellPlate 24x96DeepWellPlate
EUR 6690

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412019-2x96DeepWellPlate 2x96DeepWellPlate
EUR 675

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)

MBS412019-4x96DeepWellPlate 4x96DeepWellPlate
EUR 1230

Plasmid DNA Isolation Solutions I, II, III

10760027-1 1 Kit
EUR 85.98

SDS PAGE Kit (Teaching)

83775 15 expt. Kit
EUR 85.44
Description: Part E

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10 each
EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2 each
EUR 470.4

DNA Ligation Kit (Teaching)

95043 10 expt. Kit
EUR 37.63
Description: Part E

ExoQualiTM Exosome Isolation Kit (Plasma)

CEIK-004L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma)

CEIK-004L-30T 30 T Ask for price

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 Samples
  • 50 Samples
  • 100 Samples

FITC Conjugation Kit (Teaching)

76941 5 expt. Kit
EUR 71.5
Description: Part E

Exosome Isolation kit (for serum & plasma)

P101 10 rxn
EUR 199

Exosome Isolation kit (for serum & plasma)

P101L 40 rxn
EUR 769

Exosome Isolation kit (for serum & plasma)

P101S 2 rxn
EUR 69

HiPer® PCR Teaching Kit

HTBM016-10PR 1 unit
EUR 65.04
Description: HiPer® PCR Teaching Kit

Southern Blotting Kit (Teaching)

46037 5 expt. Kit
EUR 102.55
Description: Part E

OrgFrontier? Plasma Membrane Isolation Kit

K414-10 each
EUR 679.2

Restriction Digestion Kit (Teaching)

18450 10 expt. Kit
EUR 72.18
Description: Part E

Restriction Digestion Kit (Teaching)

18450-1 50 expt. Kit
EUR 140.95
Description: Part E

HiPer® Ligation Teaching Kit

HTBM019-20PR 1 unit
EUR 86.49
Description: HiPer® Ligation Teaching Kit

HiPer® Ligation Teaching Kit

HTBM019-5PR 1 unit
EUR 32.76
Description: HiPer® Ligation Teaching Kit

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)

CEIK-006L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)

CEIK-006L-30T 30 T Ask for price

HiPer® RA Test Teaching Kit

HTI019-20PR 1 unit
EUR 15.98
Description: HiPer® RA Test Teaching Kit

HiPer® RT-PCR Teaching Kit

HTBM024-5PR 1 unit
EUR 102.79
Description: HiPer® RT-PCR Teaching Kit

HiPer® RPR Test Teaching kit

HTI020-20PR 1 unit
EUR 14.35
Description: HiPer® RPR Test Teaching kit

HiPer® DOT ELISA Teaching Kit

HTI015-15PR 1 unit
EUR 26.94
Description: HiPer® DOT ELISA Teaching Kit

HiPer® VDRL Test Teaching Kit

HTI021-10PR 1 unit
EUR 11.64
Description: HiPer® VDRL Test Teaching Kit

HiPer® Transduction Teaching Kit

HTM005E-20PR 1 unit
EUR 158.76
Description: HiPer® Transduction Teaching Kit

HiPer® Transduction Teaching Kit

HTM005E-5PR 1 unit
EUR 44.02
Description: HiPer® Transduction Teaching Kit

Plasma Membrane Isolation Kit, Catalog: PI-0104

PI-0104 50 preparations
EUR 450

HiPer® Nested PCR Teaching Kit

HTBM043-10PR 1 unit
EUR 48.56
Description: HiPer® Nested PCR Teaching Kit

HiPer® SDS-PAGE Teaching Kit

HTP001-10PR 1 unit
EUR 48.37
Description: HiPer® SDS-PAGE Teaching Kit

HiPer® SDS-PAGE Teaching Kit

HTP001-20PR 1 unit
EUR 73.27
Description: HiPer® SDS-PAGE Teaching Kit

HiPer® InstaDNA™ Teaching Kit

HTBM033-5PR 1 unit
EUR 44.72
Description: HiPer® InstaDNA™ Teaching Kit

HiPer® Native PAGE Teaching Kit

HTP003-5PR 1 unit
EUR 119.46
Description: HiPer® Native PAGE Teaching Kit

HiPer® Transformation Teaching Kit

HTBM017E-10PR 1 unit
EUR 65.04
Description: HiPer® Transformation Teaching Kit

HiPer® Ezy MIC™ Teaching Kit

HTM006E-10PR 1 unit
EUR 72.46
Description: HiPer® Ezy MIC™ Teaching Kit

HiPer® Multiplex PCR Teaching Kit

HTBM023-5PR 1 unit
EUR 65.39
Description: HiPer® Multiplex PCR Teaching Kit

HIPER® GMO DETECTION TEACHING KIT

HTBM035-5PR 1 unit
EUR 32.76
Description: HIPER® GMO DETECTION TEACHING KIT

Bacterial Transformation Kit - 1 (Teaching)

64279 15 expt. Kit
EUR 78.77
Description: Part E

Agarose Gel Electrophoresis Kit (Teaching)

66602 15 expt. Kit
EUR 44.05
Description: Part E

Bacterial Transformation Kit - 1 (Teaching)

64279-1 30 expt. Kit
EUR 111.53
Description: Part E

HiPer® DNA Estimation Teaching Kit

HTBC006-20PR 1 unit
EUR 107.91
Description: HiPer® DNA Estimation Teaching Kit

HiPer® DNA Estimation Teaching Kit

HTBC006-5PR 1 unit
EUR 35.08
Description: HiPer® DNA Estimation Teaching Kit

HiPer® RNA Estimation Teaching Kit

HTBC007-20PR 1 unit
EUR 115.82
Description: HiPer® RNA Estimation Teaching Kit

HiPer® RNA Estimation Teaching Kit

HTBC007-5PR 1 unit
EUR 37.59
Description: HiPer® RNA Estimation Teaching Kit

HiPer® Blood Grouping Teaching Kit

HTI008-100PR 1 unit
EUR 13.7
Description: HiPer® Blood Grouping Teaching Kit

HiPer® Sandwich ELISA Teaching Kit

HTI014-4PR 1 unit
EUR 40.36
Description: HiPer® Sandwich ELISA Teaching Kit

HiPer® PCR Application Teaching Kit

HTBM030-20PR 1 unit
EUR 275.37
Description: HiPer® PCR Application Teaching Kit

HiPer® PCR Application Teaching Kit

HTBM030-5PR 1 unit
EUR 79.73
Description: HiPer® PCR Application Teaching Kit

HiPer® Phage Titration Teaching Kit

HTM003E-10PR 1 unit
EUR 84.26
Description: HiPer® Phage Titration Teaching Kit

HiPer® Immunoprecipitation Teaching Kit

HTI016-5PR 1 unit
EUR 75.78
Description: HiPer® Immunoprecipitation Teaching Kit

HiPer® Western Blotting Teaching Kit

HTI009-20PR 1 unit
EUR 274.64
Description: HiPer® Western Blotting Teaching Kit

HiPer® Western Blotting Teaching Kit

HTI009-5PR 1 unit
EUR 82.63
Description: HiPer® Western Blotting Teaching Kit

HiPer® IgG Purification Teaching Kit

HTI022-5PR 1 unit
EUR 219.05
Description: HiPer® IgG Purification Teaching Kit

HiPer® GST Purification Teaching Kit

HTP004-5PR 1 unit
EUR 156.09
Description: HiPer® GST Purification Teaching Kit

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

HiPer® Immunohistochemistry Teaching Kit

HTI023-5PR 1 unit
EUR 144.14
Description: HiPer® Immunohistochemistry Teaching Kit

HiPer® Southern Blotting Teaching Kit

HTBM027-5PR 1 unit
EUR 100.09
Description: HiPer® Southern Blotting Teaching Kit

HiPer® Northern Blotting Teaching Kit

HTBM028-5PR 1 unit
EUR 130.55
Description: HiPer® Northern Blotting Teaching Kit

HiPer® Real-Time PCR Teaching Kit

HTBM032-10PR 1 unit
EUR 101.32
Description: HiPer® Real-Time PCR Teaching Kit

HiPer® Protein Estimation Teaching Kit

HTBC004-25PR 1 unit
EUR 94.6
Description: HiPer® Protein Estimation Teaching Kit

HiPer® Protein Estimation Teaching Kit (

HTBC005-5PR 1 unit
EUR 27.88
Description: HiPer® Protein Estimation Teaching Kit (

HiPer® Restriction Mapping Teaching Kit

HTBM021-20PR 1 unit
EUR 96.52
Description: HiPer® Restriction Mapping Teaching Kit

HiPer® Restriction Mapping Teaching Kit

HTBM021-5PR 1 unit
EUR 46.1
Description: HiPer® Restriction Mapping Teaching Kit

HiPer® Latex Agglutination Teaching Kit

HTI001-20PR 1 unit
EUR 59.93
Description: HiPer® Latex Agglutination Teaching Kit

HiPer® Yeast Transformation Teaching Kit

HTBM025E-10PR 1 unit
EUR 62.08
Description: HiPer® Yeast Transformation Teaching Kit

BioLit Gene Amplification Kit - PCR (Teaching)

19317 10 expt. Kit
EUR 104.17
Description: Part E

BioLit Gene Amplification Kit - PCR (Teaching)

19317-1 25 expt. Kit
EUR 147.88
Description: Part E

HiPer® Teaching Kits-Handbook

MBTL001-1NO 1 unit
EUR 11.97
Description: HiPer® Teaching Kits-Handbook

Minute TM Plasma Membrane Protein Isolation Kit (50 Tests)

SM-005 each
EUR 555

HiPer® Gel Extraction Teaching Kit (Colu

HTBM010-10PR 1 unit
EUR 48.37
Description: HiPer® Gel Extraction Teaching Kit (Colu

HiPer® Gel Extraction Teaching Kit (Solu

HTBM011-10PR 1 unit
EUR 58.31
Description: HiPer® Gel Extraction Teaching Kit (Solu

HiPer® Bacterial RNA Teaching Kit (Colum

HTBM042-10PR 1 unit
EUR 32.63
Description: HiPer® Bacterial RNA Teaching Kit (Colum

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

HiPer® Total RNA Extraction Teaching Kit

HTBM012-10PR 1 unit
EUR 58.31
Description: HiPer® Total RNA Extraction Teaching Kit

HiPer® Cloning Teaching Kit (Blue-White

HTBM036-5PR 1 unit
EUR 83.75
Description: HiPer® Cloning Teaching Kit (Blue-White

HiPer® Widal Test Teaching Kit (Slide Te

HTI017-20PR 1 unit
EUR 18.66
Description: HiPer® Widal Test Teaching Kit (Slide Te

HiPer® Widal Test Teaching Kit (Tube Tes

HTI018-10PR 1 unit
EUR 57.61
Description: HiPer® Widal Test Teaching Kit (Tube Tes

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

Genomic DNA Extraction (from leaves) Kit (Teaching)

61498 15 expt. Kit
EUR 89.29
Description: Part E

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Total RNA Isolation Kit (Plant)

PDC07-0100 100 rxns
EUR 209

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

Exosome Isolation Kit

EIK01 2T
EUR 800
Description: Chromatography Reagents

Exosome Isolation Kit

MBS8579443-20Tests 20Tests
EUR 2625

Exosome Isolation Kit

MBS8579443-5x20Tests 5x20Tests
EUR 12240

MicroRNA Isolation Kit

KS341025 1 kit
EUR 284

Endosome Isolation Kit

P528 - Ask for price

Genomic DNA Extraction (from bacteria) Kit (Teaching)

83462 15 expt. Kit
EUR 63.97
Description: Part E

VEX Exosome Isolation Reagent (from plasma)

R603 10 ml
EUR 769.2

Microsome Isolation Kit

K249-50 each
EUR 633.6

Genomic DNA Isolation Kit (Plant)

PDC10-0100 100 rxns
EUR 132

Protoplast Isolation Kit

30210002-1 20 Prep(s)
EUR 199.16

Full genome sequence of marine Roseobacter lineage member Monaibacterium sp. ALG8 with six plasmids remoted from seawater round brown algae

Monaibacterium sp. ALG8 (=MCCC 1 Ok04733) was remoted from seawater round brown algae. The genome of Monaibacterium sp. ALG8 was sequenced, one round 3,036,380 bp chromosome and 6 round plasmids starting from 12,229 to 151,263 bp have been discovered after meeting. The outcomes of genomic annotation confirmed that Monaibacterium sp. ALG8 lacks the flexibility to degrade alginate, indicating its ecological position is probably not instantly associated to the degradation of brown algae. The comparability of genomic options within the plasmids confirmed that the majority of those plasmids, besides pALG4, have been horizontally recruited from donors, not ancestors. Primarily based on predicted features, the existence of plasmids might present pressure ALG8 with benefits together with nitrate discount, tolerance of osmotic stress by way of glycine betaine, resistance to heavy steel stress equivalent to mercury and cobalt, degradation of benzoate metabolites equivalent to p-cumate, transformation of the swim-or-stick way of life and enchancment of the immune system with two CRISPR-Cas methods.

This research offers proof for the carbon metabolic patterns of Monaibacterium sp. ALG8 and predicts the features and donors of six plasmids on this pressure, broadening our understanding of the ecological roles of micro organism within the surroundings round brown algae and the features and evolutionary patterns of plasmids in marine Roseobacter lineage members.

Genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and curing of the endogenous plasmid pBV01

Goals: To assemble a genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and determine the origin aspect in an endogenous plasmidpBV01 for curing pBV01 by plasmid incompatibility.

Outcomes: A plasmid pUBC01 was constructed, after which an electrotransformation system for B. velezensis NSZ-YBGJ001 was developed, which reached ~ 1000 transformants per microgram of pUBC01 DNA. Moreover, a 7276-bp round plasmid pBV01 with a G + C content material of 37.5% was remoted from B. velezensis NSZ-YBGJ001 and analyzed by way of sequence evaluation. To remedy pBV01, an incompatible plasmid pBV02 harboring the replication aspect of pBV01 was developed and functionally replicated in each Bacillus subtilis WB600 and B. velezensis NSZ-YBGJ001. pBV01 was cured by way of introduction of pBV02 into B. velezensis NSZ-YBGJ001 after serial subculturing for roughly 40 generations. Lastly, one other plasmid, pBV03, was constructed primarily based on pBV-ori, and exogenous genes in pBV03 could possibly be effectively expressed in B. subtilis.

Conclusions: The outcomes of this research, together with the genetic transformation system, plasmid-curing technique, and exogenous gene expression, will help genetic manipulation of B. velezensis to advertise its software in biocontrol and trade.

A high-efficiency technique for site-directed mutagenesis of enormous plasmids primarily based on giant DNA fragment amplification and recombinational ligation

Website-directed mutagenesis for big plasmids is a tough job that can’t simply be solved by the traditional strategies utilized in many laboratories. On this research, we developed an efficient technique for Website-directed Mutagenesis for Giant Plasmids (SMLP) primarily based on a PCR method. The SMLP technique combines a number of efficient approaches, together with a high-efficiency DNA polymerase for the big DNA amplification, two impartial PCR reactions and a quick recombinational ligation. Utilizing this technique, we have now achieved a wide range of mutants for the filamin A gene (7.9 kb) cloned within the pcDNA (5.four kb) or the pLV-U6-CMV-EGFP (9.four kb) plasmids, indicating that this technique might be utilized to site-directed mutagenesis for the plasmids as much as 17.Three kb.

We present that the SMLP technique has a higher benefit than the traditional strategies examined on this research, and this technique might be utilized to substitution, deletion, and insertion mutations for each giant and small plasmids in addition to the meeting of three fragments from PCR reactions. Altogether, the SMLP technique is easy, efficient, and helpful to the laboratories that require finishing the mutagenesis of enormous plasmids.

pOET-4 transfer plasmid (10ug)

GWB-282B50 0.01 mg Ask for price

pOET 2 N/C_6xHis™ Transfer Vector

GWB-001031 10 ug Ask for price

GWB-5A59EB-10UG - pOET-1 transfer plasmid (10ug)

GWB-5A59EB-10UG 10ug
EUR 287

pOET3 transfer plasmid

200104 10 µg
EUR 208.32

pOET4 transfer plasmid

200105 10 µg
EUR 208.32

pOET5.1 transfer plasmid

200106 10 µg
EUR 208.32

pOET8.VE2 transfer plasmid

200122 10 µg
EUR 409.2

pOET8.VE3 transfer plasmid

200123 10 µg
EUR 409.2

pOET9 EF1α transfer plasmid

200131 10 µg
EUR 280.24

pOET9 CCAG transfer plasmid

200132 10 µg
EUR 280.24

pOET9 SV40 transfer plasmid

200134 10 µg
EUR 280.24

pOET6 BacMAM transfer plasmid

200107 10 µg
EUR 208.32

pOET-5 Transfer Vector (10ug)

GWB-200106 10 ug Ask for price

6XHis azide

12628 1mg
EUR 301
Description: 6XHis azide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

6XHis azide

12628-1mg 1 mg
EUR 295
Description: 6XHis azide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

6XHis alkyne

12629-1mg 1 mg
EUR 295
Description: 6XHis alkyne is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

6XHis maleimide

12626-1mg 1 mg
EUR 295
Description: 6XHis maleimide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

Anti-6xHis [N144/14R] Plasmid

PVT50418 2ug
EUR 280

pOET Sequencing Primers

GWB-200100 2 x 100 ul Ask for price

pCDNA3.1-6XHis-UBD

PVT30908 2ug
EUR 280

pCDNA3.1-6XHis-UFM1

PVT30910 2ug
EUR 280

pCDNA3.1-6XHis-ISG15

PVT30913 2ug
EUR 280

pCDNA3.1-6XHis-UBE2I

PVT27974 2ug
EUR 280

pCDNA3.4-6XHis-UBE1L

PVT30453 2ug
EUR 280

pCDNA3.4-6XHis-UBE2L6

PVT30272 2ug
EUR 280

PLTP (Plasma Phospholipid Transfer Protein)

MBS613178-02mL 0.2mL
EUR 650

PLTP (Plasma Phospholipid Transfer Protein)

MBS613178-5x02mL 5x0.2mL
EUR 2765

6XHis Succinimidyl Ester

12624 1mg
EUR 301
Description: 6XHis Succinimidyl Ester is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

6XHis Succinimidyl Ester

12624-1mg 1 mg
EUR 295
Description: 6XHis Succinimidyl Ester is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

6xHis Conjugated Antibody

MBS9446006-01mLAF350 0.1mL(AF350)
EUR 480

6xHis Conjugated Antibody

MBS9446006-01mLAF405 0.1mL(AF405)
EUR 480

6xHis Conjugated Antibody

MBS9446006-01mLAF488 0.1mL(AF488)
EUR 480

6xHis Conjugated Antibody

MBS9446006-01mLAF555 0.1mL(AF555)
EUR 480

6xHis Conjugated Antibody

MBS9446006-01mLBiotin 0.1mL(Biotin)
EUR 480

pET- NLS- Cas9- 6xHis

PVT10639 2ug
EUR 215

pCDNA3.1-NKD1(mut)-6XHis

PVT29019 2ug
EUR 280

His-tag | 6xHis (polyclonal)

AS19-4255 100 µg
EUR 312

pET-(-30)GFP-9xGGS-Cre-6xHis Plasmid

PVT17107 2ug
EUR 216

OAIA00006-1MG - 6xHIS Antibody

OAIA00006-1MG 1.0mg
EUR 859

Histidine (6xHis-tag) Antibody

MBS5680102-1mg 1mg
EUR 515

Histidine (6xHis-tag) Antibody

MBS5680102-5x1mg 5x1mg
EUR 2110

OAIA00003-100UG - 6xHIS Antibody

OAIA00003-100UG 0.1mg
EUR 259

His-tag | 6xHis (100 µg, Clone AD1,1,10)

AS15-2937 100 µg
EUR 443

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free)

MBS633130-01mg 0.1mg
EUR 630

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free)

MBS633130-5x01mg 5x0.1mg
EUR 2680

N-terminal 6xHis-B2M-tag

MBS7046830-1mg 1mg
EUR 230

N-terminal 6xHis-GST-tag

MBS7046838-INQUIRE INQUIRE Ask for price

N-terminal 6xHis-Trx-tag

MBS7046849-INQUIRE INQUIRE Ask for price

N-terminal 6xHis-SUMO-tag

MBS7046828-1mg 1mg
EUR 230

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (AP)

MBS6431996-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (AP)

MBS6431996-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (PE)

MBS6432006-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (PE)

MBS6432006-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (APC)

MBS6431997-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (APC)

MBS6431997-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (HRP)

MBS6432000-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (HRP)

MBS6432000-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (FITC)

MBS6431999-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (FITC)

MBS6431999-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (Biotin)

MBS6431998-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (Biotin)

MBS6431998-5x01mL 5x0.1mL
EUR 4120

MsrB2 Protein aa: 42-182 (6xHis-tag)

MB201 2 x 50 µg
EUR 340.08

MsrB2 Protein aa: 42-182 (6xHis-tag)

MB201-XL 1 x 1 mg
EUR 1490.32

N-terminal 6xHis-B2M-JD-tag

MBS7046834-INQUIRE INQUIRE Ask for price

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 490)

MBS6199128-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 490)

MBS6199128-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 550)

MBS6209803-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 550)

MBS6209803-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 405)

MBS6188453-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 405)

MBS6188453-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 650)

MBS6220478-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 650)

MBS6220478-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 750)

MBS6231153-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 750)

MBS6231153-5x01mL 5x0.1mL
EUR 3800

His-tag | 6xHis (monoclonal, Clone 2C5,1)

AS20-4457 100 µg
EUR 367

LCK with C-tGFP tag for Plasma memberane marking (10ug transfection-grade plasmid)

RC100017 10 µg Ask for price

LCK with C-tRFP tag for Plasma memberane marking (10ug transfection-grade plasmid)

RC100049 10 µg Ask for price

CETP (untagged)-Human cholesteryl ester transfer protein, plasma (CETP)

SC124106 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 405)

MBS6432001-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 405)

MBS6432001-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 490)

MBS6432002-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 490)

MBS6432002-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 550)

MBS6432003-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 550)

MBS6432003-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 650)

MBS6432004-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 650)

MBS6432004-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 750)

MBS6432005-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 750)

MBS6432005-5x01mL 5x0.1mL
EUR 4120

His-tag | 6xHis (clone HIS,H8 / EH158)

AS11-1771 50 µg
EUR 259

H-Ras Protein; wild type 6xHis Tag

RS01-A 1 x 100 µg
EUR 469.04

H-Ras Protein; wild type 6xHis Tag

RS01-C 3 x 100 µg
EUR 836.16

ExoFectin® Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 20 rxn
EUR 499

CCND1 with C-tGFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100009 10 µg Ask for price

LAMP1 with C-tGFP tag for Lysosome marking (10ug transfection-grade plasmid)

RC100016 10 µg Ask for price

LMNB1 with N-tGFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100018 10 µg Ask for price

Rab4 with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid)

RC100025 10 µg Ask for price

Rab5 with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid)

RC100026 10 µg Ask for price

RhoB with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid)

RC100027 10 µg Ask for price

CCND1 with C-tRFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100041 10 µg Ask for price

LAMP1 with C-tRFP tag for Lysosome marking (10ug transfection-grade plasmid)

RC100048 10 µg Ask for price

LMNB1 with N-tRFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100050 10 µg Ask for price

Rab4 with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid)

RC100057 10 µg Ask for price

Rab5 with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid)

RC100058 10 µg Ask for price

RhoB with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid)

RC100059 10 µg Ask for price

LAMP1 with C-mGFP tag for Lysosome marking (10ug transfection-grade plasmid)

RC100089 10 µg Ask for price

CCND1 with C-mGFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100094 10 µg Ask for price

LMNB1 with N-mGFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100095 10 µg Ask for price

GBA2 with C-mGFP tag for Microsome marking (10ug transfection-grade plasmid)

RC100099 10 µg Ask for price

LAMP1 with C-mRFP tag for Lysosome marking (10ug transfection-grade plasmid)

RC100123 10 µg Ask for price

CCND1 with C-mRFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100128 10 µg Ask for price

LMNB1 with N-mRFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100129 10 µg Ask for price

GBA2 with C-mRFP tag for Microsome marking (10ug transfection-grade plasmid)

RC100133 10 µg Ask for price

LAMP1 with C-mBFP tag for Lysosome marking (10ug transfection-grade plasmid)

RC100157 10 µg Ask for price

CCND1 with C-mBFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100162 10 µg Ask for price

LMNB1 with N-mBFP tag for Nucleus marking (10ug transfection-grade plasmid)

RC100163 10 µg Ask for price

GBA2 with C-mBFP tag for Microsome marking (10ug transfection-grade plasmid)

RC100167 10 µg Ask for price

CLTB with N-tGFP tag for Coated pit marking (10ug transfection-grade plasmid)

RC100010 10 µg Ask for price

CLTB with N-tRFP tag for Coated pit marking (10ug transfection-grade plasmid)

RC100042 10 µg Ask for price

CLTB with N-mGFP tag for Coated Pit marking (10ug transfection-grade plasmid)

RC100071 10 µg Ask for price

CLTB with N-mRFP tag for Coated Pit marking (10ug transfection-grade plasmid)

RC100105 10 µg Ask for price

CLTB with N-mBFP tag for Coated Pit marking (10ug transfection-grade plasmid)

RC100139 10 µg Ask for price

BID with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100007 10 µg Ask for price

PLK1 with N-tGFP tag for Centrosome marking (10ug transfection-grade plasmid)

RC100023 10 µg Ask for price

PXMP2 with N-tGFP tag for Peroxisome marking (10ug transfection-grade plasmid)

RC100024 10 µg Ask for price

BID with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100039 10 µg Ask for price

PLK1 with N-tRFP tag for Centrosome marking (10ug transfection-grade plasmid)

RC100055 10 µg Ask for price

PXMP2 with N-tRFP tag for Peroxisome marking (10ug transfection-grade plasmid)

RC100056 10 µg Ask for price

PLK1 with N-mGFP tag for Centrosome marking (10ug transfection-grade plasmid)

RC100070 10 µg Ask for price

BID with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100090 10 µg Ask for price

PLK1 with N-mRFP tag for Centrosome marking (10ug transfection-grade plasmid)

RC100104 10 µg Ask for price

BID with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100124 10 µg Ask for price

PLK1 with N-mBFP tag for Centrosome marking (10ug transfection-grade plasmid)

RC100138 10 µg Ask for price

BID with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100158 10 µg Ask for price

CETP (GFP-tagged) - Human cholesteryl ester transfer protein, plasma (CETP)

RG206561 10 µg Ask for price

FtsZ Protein (E.coli recombinant, 6xHis-tagged)

FTZ05-A 1 x 1 mg
EUR 444.08

FtsZ Protein (E.coli recombinant, 6xHis-tagged)

FTZ05-B 5 x 1 mg
EUR 1958.32

ACTB with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100002 10 µg Ask for price

PDHA1 with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100006 10 µg Ask for price

PFN1 with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100022 10 µg Ask for price

PDHA1 with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100038 10 µg Ask for price

PFN1 with N-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100054 10 µg Ask for price

ATG12 with C-mGFP tag for Auophagasome marking (10ug transfection-grade plasmid)

RC100066 10 µg Ask for price

PDHA1 with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100091 10 µg Ask for price

ATG12 with C-mRFP tag for Auophagasome marking (10ug transfection-grade plasmid)

RC100100 10 µg Ask for price

PDHA1 with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100125 10 µg Ask for price

ATG12 with C-mBFP tag for Auophagasome marking (10ug transfection-grade plasmid)

RC100134 10 µg Ask for price

PDHA1 with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)

RC100159 10 µg Ask for price

GAP43 with C-tGFP tag for Neuroal axis marking (10ug transfection-grade plasmid)

RC100013 10 µg Ask for price

GAP43 with C-tRFP tag for Neuroal axis marking (10ug transfection-grade plasmid)

RC100045 10 µg Ask for price

RhoB with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100075 10 µg Ask for price

Rab4 with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100077 10 µg Ask for price

Rab5 with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100078 10 µg Ask for price

RhoB with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100109 10 µg Ask for price

Rab4 with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100111 10 µg Ask for price

Rab5 with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100112 10 µg Ask for price

RhoB with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100143 10 µg Ask for price

Rab4 with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100145 10 µg Ask for price

Rab5 with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid)

RC100146 10 µg Ask for price

OAIA00010-1MG - 6xHIS Antibody - HRP Conjugated

OAIA00010-1MG 1.0mg
EUR 879

OAIA00016-1MG - 6xHIS Antibody - HRP Conjugated

OAIA00016-1MG 1.0mg
EUR 879

His-tag | 6xHis, HRP conjugated (Clone AD1,1,10)

AS15-2930 100 µg
EUR 443

His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)

AS15-2935 100 µg
EUR 431

OAIA00007-1MG - 6xHIS Antibody - FITC Conjugated

OAIA00007-1MG 1.0mg
EUR 869

OAIA00015-1MG - 6xHIS Antibody - FITC Conjugated

OAIA00015-1MG 1.0mg
EUR 869

OAIA00005-100UG - 6xHIS Antibody - HRP Conjugated

OAIA00005-100UG 0.1mg
EUR 279

OAIA00013-100UG - 6xHIS Antibody - HRP Conjugated

OAIA00013-100UG 0.1mg
EUR 279

ATG12 with C-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)

RC100004 10 µg Ask for price

MAPRE3 with C-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100019 10 µg Ask for price

TUBA1B with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100030 10 µg Ask for price

ATG12 with C-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid)

RC100036 10 µg Ask for price

MAPRE3 with C-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100051 10 µg Ask for price

MAPRE3 with C-mGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100072 10 µg Ask for price

MAPRE3 with C-mRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100106 10 µg Ask for price

MAPRE3 with C-mBFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

RC100140 10 µg Ask for price

SYP with N-tGFP tag for Synaptic vesicles marking (10ug transfection-grade plasmid)

RC100028 10 µg Ask for price

TGOLN with C-tGFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)

RC100029 10 µg Ask for price

SYP with N-tRFP tag for Synaptic vesicles marking (10ug transfection-grade plasmid)

RC100060 10 µg Ask for price

TGOLN with C-tRFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)

RC100061 10 µg Ask for price

TGOLN with C-mGFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)

RC100088 10 µg Ask for price

PXMP2 (PTS) with N-mGFP tag for Peroxisome marking (10ug transfection-grade plasmid)

RC100096 10 µg Ask for price

TGOLN with C-mRFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)

RC100122 10 µg Ask for price

PXMP2 (PTS) with N-mRFP tag for Peroxisome marking (10ug transfection-grade plasmid)

RC100130 10 µg Ask for price

TGOLN with C-mBFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)

RC100156 10 µg Ask for price

PXMP2 (PTS) with N-mBFP tag for Peroxisome marking (10ug transfection-grade plasmid)

RC100164 10 µg Ask for price

OAIA00004-100UG - 6xHIS Antibody - FITC Conjugated

OAIA00004-100UG 0.1mg
EUR 269

OAIA00008-1MG - 6xHIS Antibody - Biotin Conjugated

OAIA00008-1MG 1.0mg
EUR 869

OAIA00012-100UG - 6xHIS Antibody - FITC Conjugated

OAIA00012-100UG 0.1mg
EUR 269

His-tag | 6xHis, Biotin Conjugated (Clone AD1,1,10)

AS15-2934 100 µg
EUR 443

MAP1LC3A with N-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)

RC100020 10 µg Ask for price

MAP1LC3B with N-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)

RC100021 10 µg Ask for price

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