Author: kathryn
Diatoms: the microscopic algae and “lungs” of the Earth that you should know
For many, it may come as a surprise, but the truth is that most of the annual oxygen production on our planet – between 50% and 85% – does not come from terrestrial forests, but from the ocean. Phytoplankton, a set of millions of microorganisms that are present in the waters that surround the five continents,…
Read MoreDiatomaceous earth is a versatile product and 100% natural, for animals and plants
The diatomaceous earth is a mineral extracted from the diatomite, which is a siliceous sedimentary rock formed by the accumulation of fossil diatoms in lakes and seas of the past. This sedimentary rock is easily extractable and sprayable and currently has a large number of uses both in industrial processes and in agriculture. The diatoms are unicellular algae microscopic belonging class of Bacilonophyta and constitute a…
Read MoreTransformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
A bacterial (neo) gene conferring resistance to neomycin-kanamycin antibiotics has been inserted into SV40 hybrid plasmid vectors and introduced into cultured mammalian cells by DNA transfusion. While normal cells die with the antibiotic G418, those that acquire and express neo continue to grow in the presence of G418. In the course of selection, neo DNA…
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Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBAD Promoter
We have constructed a series of plasmid vectors (pBAD vectors) that contain the PBAD promoter of the Arab and operon (arabinose) and the gene that encodes the positive and negative regulator of this promoter, araC. Using the phone gene and phoA fusions to monitor expression in these vectors, we show that the induction/repression ratio can…
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